New method could unleash powerful biotechnology applications
[ad_1] Design and testing of CRISPRi knockdowns to assess gene essentiality in phages lambda and P1. (a) Schematic of CRISPRi assay system. (b) Representative images of plaque assays to validate the dCas12a CRISPRi system using gene targets with known essentiality. We employed crRNAs targeting 2 essential genes of phage λ: genes encoding major capsid protein […]
